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CCI – Centre for Cell Imaging

Hardware issues

DEMO Microscopes from 17th-22nd of May

We are testing different microscopes to replace the Leica SPE2 “workhorse” that is having frequent technical failures and is heavily overbooked nowadays. Our aim is to increase the imaging capacity at the CCI by improving the workflow to do your imaging. We sent a mail with a request to give us samples you would like…

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Leica SPE2 40x HCX oil objective defect

The 40x oil on the Leica SPE-II CLSM showed severe chromatic aberration in Z for UV fluorescence. The objective is removed from the system and its aberration is being validated on another Leica machine in the UU campus. We probably need to repair or replace it. Our objectives are very costly and vulnerable, please handle…

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Update NIKON A1R upcoming

We are grateful to the Veterinary Faculty Utrecht to support an upgrade for the NIKON  A1R system. Many users will benefit from this upgrade since it will vastly improve confocal imaging speeds. The upgrade will be performed  around the last week of May 2019.

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Soledad appointed, Cryostate moved and supported by Judith in NG O.53

Soledad Ordoñez-Alvarez has been appointed for 2 days a week as a new staff member of the CCI. She specializes in instructing new users and adds expertise in image analyses to the CCI. Unfortunately, Rob Bleumink had to retire. Since we miss his expertise in cryosectioning (as for countless other issues), we moved the cryostate…

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OMX service performed CAM4 currently not functional

The OMX has been serviced , the OTFs have been updated and saved as default for SIM reconstructions. A 10x objective is available now to perform tile scans for sample overviews. The 4th camera is non functional due to a power failure, repair parts are on order. So for now, the far red detection is…

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Joystick arrived for OMX V4

A newly developed joystick is installed on the OMX-V4, it allows navigating through a sample, mark and start acquisition/mosaic. It is a bit fast but with practice will help you find the best cells more efficiently. Few new features are implemented during the last service visit (for example: laser power tuning), ask CCI staff to…

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TOKAI hit lens heater working again

The TOKAI HIT heater for lenses on the NIKON system is repaired. Please be careful wrapping the foil around the lenses, the fit should be tight but do not pull the wiring or deform the aluminium foil while wrapping.

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OMX service has been performed

The OMX system has been serviced and new OTFs have been generated. The current OTF set that is selected  in SoftWoRx should be used for reconstructions, do not change it!  

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