Logo Utrecht University

CCI – Centre for Cell Imaging

Hardware issues

Leica SPE2 40x HCX oil objective defect

The 40x oil on the Leica SPE-II CLSM showed severe chromatic aberration in Z for UV fluorescence. The objective is removed from the system and its aberration is being validated on another Leica machine in the UU campus. We probably need to repair or replace it. Our objectives are very costly and vulnerable, please handle them with care. A microcourse on how to prevent lens damage is given by dr. Waters (link). We notice a great number of scratches on the top beveled end of all lenses, please realize that these are often generated by collisions to the bottom edge of a sample holder. Please make sure lenses are lowered before rotating the turret and realize that moving the stage at some point will get the lens too close to the sample holder edge.