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CCI – Centre for Cell Imaging

Leica SPE-II

DEMO Microscopes from 17th-22nd of May

We are testing different microscopes to replace the Leica SPE2 “workhorse” that is having frequent technical failures and is heavily overbooked nowadays. Our aim is to increase the imaging capacity at the CCI by improving the workflow to do your imaging. We sent a mail with a request to give us samples you would like…

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Leica SPE2 40x HCX oil objective defect

The 40x oil on the Leica SPE-II CLSM showed severe chromatic aberration in Z for UV fluorescence. The objective is removed from the system and its aberration is being validated on another Leica machine in the UU campus. We probably need to repair or replace it. Our objectives are very costly and vulnerable, please handle…

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Walking directions during reboot of the CCI labs

Working on the Leica SPE2: Entry: General entrance AG East 1 st floor, direct right turn to access the Confocal room O.161, knock before entry and apply to the hygiene rules that are supplied. A second person can be present in the room yet distance of 1.5 m should be kept at all times. Exit:…

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Missing 3.5 cm dish holder for SPE-2 confocal

We are missing the 3.5 cm dish holder that allows clamping a 3.5 dish onto the galvano stage of the SPE-2 confocal. Please return if you used it, other people need to use it!! It should be stored in the left cabinet behind the SPE-2 above the freezer. 

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April 4: ImageJ Advanced workshop

The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific…

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Curtains around SPEII

Finally, we have curtains around the SPEII to seal the microscope space from the rest of the lab. This way we now can work normally in the wetlab again with the lights on.

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Leica SPEII vibration issue

Image distortions due to vibrations on the system were reported last week. We elevated the vibration insulator silicon supports and temporarily solved the problem. New insulators will be placed during the next Leica service. Please notify us when stripe patterns appear in your images.

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Leica SPE-II shifted in room O-101

We have an old building that is leaky, a serious water spill occurred when the last storm hit the east wing of the Androclus building. The equipment (the SPEII) was not damaged due to the swift action of Martijn Moolenaar (thanks again). Apparently, the rain leaked into the building at multiple departments and it is a…

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