CCI – Centre for Cell Imaging

Hardware issues

Lens heater on NIKON A1R/STORM again failing

Unfortunately, the TOKAI hit lens heater is broken again. We are in contact with the Japanese supplier but it may take some time for it to be repaired (it needs to be sent to Japan). We have imaged live cells without the heater overnight without any problems, yet the temperature at the lens spot will…

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Objective heater Nikon is replaced

The TOKAI hit objective heater is complete again. Be careful when changing objectives when the velcro is around it. The connecting wires can be dislodged from the connectors by these fast movements. Also, the aluminium inner surface is fragile be carefull when you wrap te velcro around an objective.

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TOKAI Hit Objective Heater NIKON defect

The objective heater for live cell imaging on the NIKON A1R-STORM system is broken. The thermocouple is being replaced. For short term time lapse (and vibration insensitive imaging) you can use the hair dryer to warm the lower part of the microscope.

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Missing 3.5 cm dish holder for SPE-2 confocal

We are missing the 3.5 cm dish holder that allows clamping a 3.5 dish onto the galvano stage of the SPE-2 confocal. Please return if you used it, other people need to use it!! It should be stored in the left cabinet behind the SPE-2 above the freezer. 

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5x plan-apochromat and 96 well PECON universal plate adapter

We obtained a 5x Plan ApoChromat lens for the Zeiss NLO-LSM880 together with a universal stage adapter from PECON (KM) that fits 85.5x 127.75 mm culture plates (i.e. 6, 12,24,48,96,384 well culture plates). Automated live cell multiwell analyses at 37 degrees  C will be possbile but without CO2 and humidity control for now. The old slider…

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NIKON TOKAI hit adapter screws replaced by magnets

The attachment of the different adapters for the TOKAI hit heating chamber on the A1R NIKON / STORM microscope has been changed.  Magnets have been glued onto the MCL-piezo stepper adapter to snap sample holders by pairing magnets. Place a sample holder and carefully put the supplied separate 12 mm diameter plate magnets over the front-left…

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OMX service has been performed, GUI bug workaround

The OMX has been serviced and new OTFs have been made. We recommend using these. A strange bug in the acquisition software causes the 405 nm laser line (1st channel) to present wrong values, i.e. intensity % of the laser in the drop-down list do not represent the actual values. The desktop background picture of…

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