CCI – Centre for Cell Imaging

Hardware issues

New Leica 40x oil immersion objective

A new objective for the SPE2 system has arrived (HC PL APO CS2 NA1.3, oil immersion). It is  installed but not fully funtional yet, fluorescence transmission is way better than the old 40x. However, optimal DIC imaging with this objective is not possible yet, we are waiting for the correct condensor prism for this.

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Move of Olympus/Leica system to O123A

An unfortunate accident forced the move of the Olympus/Leica system to the corridor next to our offices (Room O123A). The old room (O122) needs a good (airco) cleaning session before new equipment can be placed.  The imaging system is fully serviced and functional at its new location.  

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New equipment coming

We are pleased to tell about the new equipment that is ordered and on its way to the CCI. Thanks to the generous funding of the Utrecht University Board to support facilities in the Utrecht Science community, we are able to upgrade the CCI with an OMX-V4 blaze. It again is the collaboration between the Stoorvogel…

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Frequent crashing LEICA SPEII

The LEICA SPEII system has serious PC issues with regular crashes (system freeze while scanning, panel box loss and the never ending story on the 561 nm laser that starts ever so slowly) . Make sure you save often and report to us when it happens. We are in the process of replacing the PC,…

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AFM moved to chemistry department

The Atomic Force microscope has been moved to the chemistry department. The group of Igmar Swart who works on the chemistry and physics of 2D materials (www) is dedicated to AFM imaging assuring frequent operation  and service of the system. Contact Igmar, Joost van der Lit or Bart Gadella if you have imaging questions for this microscope.

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LEICA SPE-II CRASH

Last Thursday, the 30th of October, the PC of the Leica SPE-II confocal crashed, this is already the second crash of the PC this year (we had the HD replaced just a half a year ago). We are trying to get the system running again in the next few days, but rely on ICT support…

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New automated diascopic shutter for DIC imaging on the A1R-setup

We bought a new shutter to rapidly block halogen light in time recordings using the EM-CCD camera of the NIKON imaging station. Previously, intervals between fluorescent observation and DIC recording required warming up the halogen lamp for at least 4 seconds. Now the light path is rapidly switchable in milliseconds and is incorporated into the…

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Hard Disk crash on the Leica SPEII system

This week (Tue 8/7/2014) the hard disk of the SPEII system crashed. We had to remove it and re-install windows. The data on the extracted disk is very poorly readable and thus data and settings of users are essentially lost. Since all users are highly disciplined in their data backup procedures (?!) we can easily retrieve…

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Leica service

On April 2nd, a Leica service engineer will try and fix the 561 nm laserline that very slowly starts. Also, the galvo x- and y- motors are going to be checked, they make strange sounds when stopping a scan, a recurring defect it seems.

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