Today, the NIS elements software on the NIKON A1R/STORM system has been updated to the newest version for the NIS suite (4.5) and the STORM module (4.1). In STORM,  Z stacking features, fiducial marker integration and sequential recordings on identical positions can be performed, potentially on regions in  live cells. In addtition, the 405 laser…

On the OMX imaging system, channel alignment is repeatedly disturbed by incorrect placement of the automated filter drawer (which sets in every time at startup) . A GE scientist is coming to solve this issue (in the week of February the 29th). Until then the filter drawer will be fixed to the BGR position to maintain…

We are experiencing problems with our netstorage. The central storage is currently not available so you will not be able to upload or download data. The data are secured and will be available again soon. Watch this blog for updates.  

The broken master PC on the OMX V4 system has been replaced and the OMX is fully functional again.

One of the PCs of the OMX-V4 system has a corrupted hard drive and does not boot. The system is not usable and will be repaired as soon as possible. The PC does not seem to be in stock in Europe, so it has been ordered in the US. As a result the system will…

The OMX V4 system is now updated with a DIC option to monitor ‘cell fitness’ or provide overviews of the cells that are imaged. So far, the quality is mediocre but we are trying to improve this.

The storage for gas tanks (CO2, N2) in the CCI corridor has been replaced and moved to make space for the flow cabinet that will be positionned in the recess behind it. Beware that the corridor has become more shallow because of the move.

The TIRF module experienced a problem yesterday and was repaired this morning. All is functional again. The NIDAQ triggering of the EM-CCD camera no longer needs manual switching of BNC connectors, NIKON made a connector box that allows automated switching from within NIS elements. So triggering via the lasers (LU4) or wide field illumination (SpectraX)…

A StorSimple backup system has been  implemented by ITS.  We have tested it for a couple of months and it seems to perform well. The extra network drive is available on all imaging machines (except for the Zeiss NLO system, that will be connected to the net in the coming weeks) and can be found…

Next week, we will be trained by GE healthcare again, now to improve alignment and calibration procedures as well as the DLM module to perform dSTORM type of measurements (alike the NIKON system). Unfortunately, only one of the 4 cameras can be used to perform DLM, Furthermore the system will be upgraded with DIC and…