CCI – Centre for Cell Imaging

Software

Update Leica handout

Esther has updated the Leica handout for software and hardware changes in the last year, please find the new version on the equipment page or here

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Update STORM software

New STORM analyses software to perform peak detection by multifitting procedure (described in: Babcock et al 2012 Opt Nanoscopy 1:6 ) is now implemented on our machines (workstation and acquisition PC).

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Update NIKON STORM software

The STORM module of the NIKON A1R/STORM system has been updated. Now 3D color chromatic aberration correction is included in the module and post acquisition filtering of peak minima and maxima has been implemented.  The workstation software is upgraded as well.

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JOBS training

We had a training session at NIKON to work with the wizard creator ‘JOBS’ within the NIS software. It is aimed to easily guide people through an imaging session. Especially multiwell plate definition, random position imaging within wells, autofocus, different image capture for different wells  can be predefined and structured. Of course, users still need to…

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Bioimaging Utrecht Winter meeting 21 01 2014

Tuesday  (21st of January), the BioImaging Utrecht community will have an open meeting on nanoscopy. Join to hear the latest on superresolution. Flyer can be obtained here. An update of the A1R software and the STORM software has been performed, functionally the 4.2 NIS version is identical (bugfixes) but the STORM module has improved possibilities for…

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Secundary Antibodies supply discontinued

We are going to discontinue the service to provide secundary fluorescently labelled antibodies. It was useful to maintain a relatively fresh stock that is continuously probed for functionality, this way users did not need to worry about out-of-date fluorescent antibodies. However, this service is too expensive to keep, and if users keep stocks as 50%…

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CCI app launched as html-app

To help users I have developed a HTML app (see QRcode below, for now- we are still in testing phase) to log mic setings and send to the user by mail, booking is also included and a checklist to guide STORM imaging is in there as well. Handy for keeping track of some settings you…

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